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. 1997 May 19;185(10):1753–1758. doi: 10.1084/jem.185.10.1753

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Expression of transgenes. (A) Flow cytometric analysis of spleens of transgenic mice. Cells were stained with FITC-conjugated anti-D1.3 idiotype and PE-conjugated anti-B220 and gated for propidium iodide exclusion. Numbers indicate percentage of splenocytes in each quadrant (Non-tg, nontransgenic). (B) Transgene expression on T cells. Splenocytes were stained with FITC-conjugated anti-IgM, biotinylated anti-Thy1.2, and PE– streptavidin; the profiles present the IgM staining of Thy1.2⁺ gated cells.