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. 1997 May 19;185(10):1851–1858. doi: 10.1084/jem.185.10.1851

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Galectin-1–induced apoptosis of thymocytes was enhanced by preincubation with CD3 antibody, but not by preincubation with dexamethasone. Thymocytes were preincubated with medium (A), 20 μM dexamethasone (B), or with 1 μg/ml CD3 antibodies (C) for 16 h before a 5-h exposure to DTT alone, or to galectin-1 plus DTT. The percentage of apoptotic cells was determined by flow cytometry after TUNEL staining. The results shown are representative of duplicate determinations.