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. 1997 May 19;185(10):1837–1849. doi: 10.1084/jem.185.10.1837

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Th1 effectors undergo more rapid apoptosis with and without restimulation. Th1 and Th2 effector cells at 5 × 10⁵ per culture were stimulated with 1.7 × 10⁵ of APC in 1 ml of medium with or without PCCF (5 μM) for 1, 2, 4 and 7 d. At each time, cultures were harvested and stained with TdT and CD4 as above. The appearance of TdT⁺ CD4 cells in Th1 (triangles) and Th2 (circles) effector cultures stimulated with antigen and APC (closed) or with APC without antigen (open) was determined. The results are the representative of three experiments.