Skip to main content
. 1997 Jun 2;185(11):1977–1985. doi: 10.1084/jem.185.11.1977

Figure 2.

Figure 2

Figure 2

Effect of specific receptor ligation on the LPS- induced expression of cytokine mRNA. (A) BMMφ from BALB/c mice were exposed to LPS (50 ng/ml), or LPS in combination with either E-IgG, E-C3bi, E-ML-BSA, or latex microspheres. 6 h after the addition of stimuli, total RNA was isolated and used to carry out competitive RT-PCR, using primers for HPRT, TNF-α, IL-10, or the inducible p40 subunit of IL-12, as described in Materials and Methods. (B) cDNA generated from BALB/c BMMφ exposed to LPS (50 ng/ml) or LPS in combination with E-IgG, were first normalized for HPRT levels. Constant volumes of normalized cDNAs were then amplified in the presence of decreasing concentrations of competitor (PQRS), using primers for the inducible p40 subunit of IL-12. The concentration of the experimental cDNA is represented by the equivalent intensities of competitor and wild-type bands. The fold decrease in IL-12(p40) levels between BMMφ exposed to LPS or LPS in combination with E-IgG can be determined by taking the ratio of their equivalence points.