Figure 3.
Effect of antibody cross-linking of BMMφ receptors on the LPS-induced expression of cytokine mRNA. BMMφ from BALB/c mice were incubated with the mAb M1/70 (anti–Mac-1) or mAb 2.4G2 (anti-CD16/32) for 15 min. After one wash with complete medium, LPS (50 ng/ml) was added to all wells. Goat anti-rat IgG F(ab′)2 was added simultaneously with the LPS to mAbtreated wells. 6 h after the addition of stimuli, total RNA was isolated and used to carry out competitive RT-PCR, using primers for HPRT, TNF-α, IL-10, or the inducible p40 subunit of IL-12, as described in detail in Materials and Methods. Results are representative of two separate experiments.