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. 1997 Jun 2;185(11):1919–1927. doi: 10.1084/jem.185.11.1919

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Substitution of D for E at the DOα chain NH₂ terminus lowers the affinity of cell-free α/β-TCR for IA^d-OVA. Various concentrations of IA^d-OVA were injected in flow cells with immobilized DOα/β TCRs bearing DOα chains with either D or E at the NH₂ terminus as described in the Materials and Methods. A flow cell with immobilized free β chain from this receptor was used as a control for signal from protein in solution and buffer differences.