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. 1997 Jun 2;185(11):1997–2013. doi: 10.1084/jem.185.11.1997

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Sialomucins on CD8 T cells can mask the recognition of 9-O-acetylated gangliosides by CHE-FcD probe. (A) Mouse spleen cells were stained with CHE-FcD before or after OSGPase or trypsin treatment and staining profiles were traced from gated CD4 and CD8 subpopulations. Trypsin treatment was done on CD4 and CD8 T cells that were pre-sorted on a FACSorter^®. Loss of binding of anti-Mel14 was a positive control for trypsinization (data not shown). (B) Mouse thymocytes were treated with OSGPase as described earlier, washed and stained with anti-CD4, CD8, and CHE-FcD. Sham treated cells were incubated with an equal volume of buffer under identical conditions. 100,000 live events were acquired and CD4 and CD8 SP cells were gated from a CD4/CD8 dot plot. CHE-FcD binding within those populations was plotted in the histograms. The dotted line shows background staining with the second antibody.

Sialomucins on CD8 T cells can mask the recognition of 9-O-acetylated gangliosides by CHE-FcD probe. (A) Mouse spleen cells were stained with CHE-FcD before or after OSGPase or trypsin treatment and staining profiles were traced from gated CD4 and CD8 subpopulations. Trypsin treatment was done on CD4 and CD8 T cells that were pre-sorted on a FACSorter^®. Loss of binding of anti-Mel14 was a positive control for trypsinization (data not shown). (B) Mouse thymocytes were treated with OSGPase as described earlier, washed and stained with anti-CD4, CD8, and CHE-FcD. Sham treated cells were incubated with an equal volume of buffer under identical conditions. 100,000 live events were acquired and CD4 and CD8 SP cells were gated from a CD4/CD8 dot plot. CHE-FcD binding within those populations was plotted in the histograms. The dotted line shows background staining with the second antibody.