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. 1997 Jun 2;185(11):1997–2013. doi: 10.1084/jem.185.11.1997

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CD4 and CD8 distribution on total thymocytes and on 9-Oacetyl⁺ cells. Three-color flow cytometry was done after staining with CHE-FcD, anti-CD4, and anti-CD8. The 9-O-acetyl positive cells were gated to obtain a selective CD4/CD8 profile. Since CD8 SP and CD4⁻ CD8⁻ DN cells constitute a low percentage of total thymocytes, 100,000 total events were acquired to analyze the small percentage of total 9-Oacetyl⁺ thymocytes.