Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1997 Jun 2;185(11):1997–2013. doi: 10.1084/jem.185.11.1997

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Relationship of 9-O-acetylation to expression of TCR, HSA, CD69 and CD5 on CD4⁺ thymocytes. Three-color staining was done with anti-CD4 and CHE-FcD along with either anti-CD3, J11D (anti-HSA), anti-CD69 or anti-CD5 as the third color. Binding of antiCD3 mAb 2C11 was detected using phycoerythrin-conjugated goat anti– hamster IgG and mAb J11D was detected by FITC conjugated donkey anti–rat IgG. Anti-CD69 and anti-CD5 were directly conjugated to FITC and anti-CD4 to tricolor reagent. Only CD4⁺ thymocytes were gated to show the dot plots relating 9-O-acetylation with other thymocyte markers. Quadrant boundaries were determined based on the background staining profiles obtained by the secondary antibodies for each axis.