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. 1997 Jun 2;185(11):1997–2013. doi: 10.1084/jem.185.11.1997

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Association of other markers with 9-O-acetylation among CD4 peripheral lymphocytes. Flow cytometry was done after three color staining using anti-CD4 and CHE-FcD along with anti-Mel14, anti-Pgp1 or anti-CD45R(exonB). All peripheral T cell markers were used as biotinylated antibodies and their binding was detected using streptavidin conjugated FITC. The data displayed here represents only CD4⁺ gated cells. Staining on each axis of the dot plot was determined after using the secondary fluorescent conjugated reagent alone without the primary antibody or probe.