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9-O-acetylated gangliosides are present in purified lipid extracts from CD4 T cells. Lipid extracts containing gangliosides from resting or activated CD4 cells were dried on to 96-well flat-bottomed plates and CHE-FcD was allowed to bind with or without base treatment (0.1 N sodium hydroxide for 15 min) to remove 9-O-acetyl groups. Binding was detected with HRPO-conjugated second antibody and substrate. Results shown are mean values from triplicates.
