Figure 9.

CHE-FcD does not affect T cell activation mediated by PMA or by cross-linking with anti-CD3 antibodies. Total cells from mouse lymph nodes were activated with varying amounts of PMA, or by cross-linking with increasing amounts of anti-CD3 antibody. [³H]thymidine incorporation into the cells measured the proliferative response of the T cells. The effect of adding CHE-FcD or CHE-Fc in solution (final 50 μg/ml in all the wells) on the dose-response curve was plotted. Values represent the mean of triplicate values. Background values for sham treated cells were consistently lower than the smallest stimulating dose (not shown).