Table 1.
>T and B Lymphocyte Differentiation Is Normal in IL-10 Transgenic Mice
| Mouse type | Cell no. | CD4+ | CD8+ | CD4+/CD8+ | B220/IgM+ | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Thymus | % | % | % | % | ||||||
| Control No. 1 | 1 × 108 | 9.8 | 2.6 | 83.7 | – | |||||
| Control No. 2 | 1.2 × 108 | 10.9 | 2.6 | 82.7 | – | |||||
| IL-10 Tg No. 1 | 9.8 × 107 | 10.4 | 3.2 | 82.1 | – | |||||
| IL-10 Tg No. 2 | 1.6 × 108 | 15.3 | 3.4 | 77.4 | – | |||||
| Spleen | ||||||||||
| Control No. 1 | 4.8 × 107 | 26.6 | 17.5 | <1 | 60.8 | |||||
| Control No. 3 | 5.5 × 107 | 22.8 | 9.7 | <1 | 61.8 | |||||
| Control No. 4 | 5.4 × 107 | 19.5 | 14.4 | <1 | 60.3 | |||||
| IL-10 Tg No. 1 | 3.0 × 107 | 22.8 | 13.2 | <1 | 62.8 | |||||
| IL-10 Tg No. 2 | 3.0 × 107 | 24.9 | 13.2 | <1 | 58.4 | |||||
| IL-10 Tg No. 3 | 6.5 × 107 | 16.5 | 10.3 | <1 | 63.7 | |||||
| IL-10 Tg No. 4 | 6.5 × 107 | 23.4 | 14.6 | <1 | 50.5 |
Cells from spleen and thymus of line 9 IL-10 transgenic (Tg) mice were prepared, counted, and stained with the various cell-surface markers. Stained cells were analyzed by flow cytometry as described in Materials and Methods. Values are given as percentages of total gated lymphocyte populations, based on light scatter. –, not done.