Table 1.

>Quantification of BSA–gold Particles Transferred from Endosomes to Leishmania-containing Phagosomes

		5-nm BSA–gold particles		16-nm BSA–gold particles
Wild type		57.3 ± 28.0		4.3 ± 2.7
C3PO		323.6 ± 142.5		23.6 ± 11.4
C3PO+LPG2		86.2 ± 34.4		8.2 ± 3.5

J774 macrophages were infected as described in the legend to Fig. 1. Cells were then fed a 5-nm and 16-nm BSA–gold particles mixture for 30 min, followed by a chase period of 60 min, and processed for electron microscopy. The 5- and 16-nm gold particles present in the profile section of phagosomes containing the various Leishmania lines were counted. At least 25 phagosomes were analyzed for each cell line. Phagosomes devoid of BSA–gold particles were not included in this quantitative analysis.