Figure 3.

LCMV-specific immune response of aly/aly mice after adoptive transfer (AT) of naive or LCMV-primed B6 spleen cells. Recipient mice were infected with 2 × 10⁵ LCMV-WE into the footpads. 3 h later, 5 × 10⁷ naive or day 8 LCMV-WE–primed B6 spleen cells were adoptively transferred. ▵, aly/aly recipients, adoptively transfused with naive B6 spleen cells; ▿, aly/aly recipients transfused with day-8 LCMVprimed B6 spleen cells; □ aly/+ recipients transfused with day-8 LCMVprimed B6 spleen cells; ▪ aly/+ recipients transfused with naive B6 spleen cells. The footpad swelling reaction was monitored daily (A). Means and SD are given for both hind footpads of two to three mice per group in a 0.1-mm scale. The CTL response was measured on day 9 after infection (B). The spleen cells were restimulated with irradiated LCMV-infected spleen cells for 5 d. Cultures were then tested on LCMV-infected ⁵¹Cr-labeled MC57G cells. Unspecific lysis of uninfected targets was <10%. Each line represents the mean of two to three mice. LCMV titers in spleen and liver on day 9 after infection (C). Single values of individual mice, means (horizontal bars) and the detection limit (det. lim.) of the assay are indicated. Values are given as log₁₀(PFU per organ). The data represent one out of two comparable experiments.