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. 1997 Jun 16;185(12):2079–2088. doi: 10.1084/jem.185.12.2079

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Ly49A transgene expression causes an MHC-dependent reduction in the levels of endogenous Ly49A mRNA. RNase protection assay of Ly49A mRNA levels and control NKRP1C levels in RNA preparations from NK cell (A-LAK) populations. One complete experiment and the H-2^d data from a second experiment are presented. The H-2^d transgenic sample in experiment 1 (lane 6) was somewhat underloaded compared to the other samples, as shown by the lower intensity of the NKR-P1C band. When the phosphorimager values for the Ly49A endogenous band were normalized for NKR-P1C levels, comparable results to the other two experiments were obtained (see Table 3), and the Ly49A transgene band was comparable to the other transgenic samples. The protected RNA fragments are 379 nt for the endogenous Ly49A, 120 nt for the Ly49A transgene, and 239 nt for NKR-P1C. The 155-bp band present in all samples is from the NKR-P1C probe, and may represent transcript for another NKR-P1 isoform. The endogenous Ly49A mRNA levels in Ly49A transgenic H-2^d NK cells are significantly reduced compared to the levels in the nontransgenic littermate, based on a P <0.01 value using the two-tailed Student's t test (Table 3). m, markers.