Table 1.

>Expression Levels of Ly49 Receptors in Ly49A Transgenic Mice

		MHC		Mean Fluorescence Intensity
				mAb JR9- 318 (Ly49A)		mAb SW5E6 (Ly49C/I)		mAb 4D11 (Ly49G2)
Tg line No. 2		β2m−/−		97 ± 9		55 ± 4		19 ± 2
		H-2b		94 ± 12		49 ± 2		19 ± 1
		H-2d		35 ± 4 ‡		36 ± 2‡		10 ± 1‡
non-Tg LM		β2m−/−		109 ± 22		56 ± 1§		19 ± 4
		H-2b		105 ± 7		48 ± 3		18 ± 1
		H-2d		48 ± 4*		39 ± 3§		12 ± 1‡
Tg line No. 12		H-2b		30 ± 4		40 ± 1		16 ± 1
		H-2b/d		13 ± 1 ‡		33 ± 2*		11 ± 1*
non-Tg LM		H-2b		90 ± 3		46 ± 3		16 ± 2
		H-2b/d		36 ± 2*		37 ± 3‡		11 ± 1‡

Values represent the means and SDs of three determinations. The nontransgenic samples were from littermates of the respective transgenic lines, analyzed in parallel. The two transgenic lines were tested in separate experiments. In the case of Ly49A transgenic mice, Ly49A staining intensity was determined by gating on all NK cells. In the case of nontransgenic littermates, Ly49A staining intensity was determined by gating on Ly49A⁺ NK cells. Substantial effects of MHC on transgene-directed Ly49A levels are indicated in bold. LM, littermate; Tg, transgenic.  

^* P <0.001;  

^‡ P <0.01;  

^§ P <0.05, compared to H-2^b mice of same type by Student's t test.