Table 3.

Cytokine Secretion by Splenocytes from Aerosol-treated Mice to 40 μg/ml of Mouse Insulin II B Chain Peptide (Amino Acids 9–23)

Cytokine		Insulin aerosol		OVA aerosol
(pg/ml)
IL-2		200 ± 15		186 ± 27
IFN-γ		368 ± 71		423 ± 31
IL-4		36 ± 6		Not detectable
IL-10		222 ± 149		Not detectable
TGF-β		293 ± 131		162 ± 41

Supernatants from the replicate culture wells (Table 2) were sampled after a 3-d incubation and were assayed for cytokines as described in Materials and Methods.