Table 1.
Target cell | ||||||||
---|---|---|---|---|---|---|---|---|
OLs | U251 glioma | |||||||
Effector | Trypan blue uptake | PI nuclear stain | Trypan blue uptake | PI nuclear stain | ||||
FasL | 56 ± 2% | 3 ± 1% | 78 ± 9% | 69 ± 8% | ||||
Yeast supernatant | 4 ± 1% | 1 ± 0.4% | 5 ± 1% | 1 ± 1% | ||||
Culture medium | 1 ± 0.2% | 2 ± 1% | 2 ± 0.4% | 2 ± 1% |
Data indicate the percentage ± SEM of triplicate experiments of unfixed OLs or glioma cells, which take up trypan blue or shown nuclear condensation or fragmentation after fixation and staining with PI in response to a 24-h exposure to fasL (1:10 dilution). Controls were either yeast medium used to prepare the fasL or culture medium alone.