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. 1996 Dec 1;184(6):2301–2310. doi: 10.1084/jem.184.6.2301

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Immediate differentiation of cultured c-Kit⁺Mac-1^dull cell in medium-containing serum. Unfractionated BM cells were cultured on OP9/erbB2, as described in the legend to Fig. 3. 21 d later, the cells were transferred onto a fresh OP9 stroma cell layer with medium-containing serum (RPMI 1640 with 10% FCS and 5 × 10⁻⁵ M 2-ME). The recovered cells were analyzed after staining with FITC-conjugated Mac-1 and biotinylated anti–c-Kit (ACK4), or FITC-conjugated anti-B220 and biotinylated anti–IL-7 receptor (A7R) (A) before, (B) 7 d, or (C) 14 d after the transfer. Biotinylated antibody was detected with streptavidin-PE. The percentage of cells in each quadrant is shown in the top right-hand corner of each panel.