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. 1996 Dec 1;184(6):2371–2384. doi: 10.1084/jem.184.6.2371

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Summary of MBP–NP and –GP transgenic mouse lines. (A) pMBP001 contains 1.9 kb of the MBP gene promoter/enhancer sequences, and polyadenylation and splice signals from the PLP gene. The LCMV NP and GP genes were cloned into the BamHI site of pMBP001 to give pMBP–NP and pMBP–GP. Expression of spliced mRNA in transgenic mice was determined by RT-PCR using primers that hybridized within the transgene and the PLP exon 7 as indicated by arrows, with the resulting RT-PCR products of 510 bp (MBP–NP) or 780 bp (MBP–GP). (B) RNA from the brains (Brn) and spleens (Spl ) of MBP– NP and –GP transgenic mice was subjected to RT-PCR as described in Materials and Methods to detect transgene-specific spliced RNA (see A). M, molecular weight markers. (C) The transgenic mouse lines studied are listed with the relative copy number of the transgene and the results of RT-PCR transgene mRNA studies.

Summary of MBP–NP and –GP transgenic mouse lines. (A) pMBP001 contains 1.9 kb of the MBP gene promoter/enhancer sequences, and polyadenylation and splice signals from the PLP gene. The LCMV NP and GP genes were cloned into the BamHI site of pMBP001 to give pMBP–NP and pMBP–GP. Expression of spliced mRNA in transgenic mice was determined by RT-PCR using primers that hybridized within the transgene and the PLP exon 7 as indicated by arrows, with the resulting RT-PCR products of 510 bp (MBP–NP) or 780 bp (MBP–GP). (B) RNA from the brains (Brn) and spleens (Spl ) of MBP– NP and –GP transgenic mice was subjected to RT-PCR as described in Materials and Methods to detect transgene-specific spliced RNA (see A). M, molecular weight markers. (C) The transgenic mouse lines studied are listed with the relative copy number of the transgene and the results of RT-PCR transgene mRNA studies.