Figure 2.

TCR gene rearrangements in the thymocytes detected by PCR. Rearrangement of TCR γ (A), δ (B), and β (C) genes. The DNA from thymocytes of fetuses at day 17 of gestation (for Vγ3–Jγ1, Vγ4– Jγ1, and Vδ1–Jδ1 rearrangements) and at 4 wk old (for Vγ1.1–Jγ4, Vγ1.2–Jγ2, Vγ2– Jγ1, Vγ5–Jγ1, Vδ4–Jδ1, Vδ5– Jδ1, and all the β gene rearrangements) was amplified by PCR, and the Southern blots of the products were hybridized with oligonucleotide probes. Combination of primers used are shown on the left side (A and B). Dβ–Jβ2 and Vβ8.2–Dβ–Jβ2 rearranged fragments are shown on the left side (C).