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. 1996 Dec 1;184(6):2411–2416. doi: 10.1084/jem.184.6.2411

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C2-ceramide inhibits presentation of native antigen but does not inhibit presentation of peptide and alloantigens. (A) Proliferative response of CD4⁺ T cell clone P6.2 to different concentrations of PjE. DCs were left untreated (open squares), pretreated with 80 μM C2-ceramide (closed circles), or with C2-dihydroceramide (open circles). (B) Proliferative response of CD4⁺ T cell clone KB24 to different concentrations of soluble TT. DCs were left untreated (open squares), pretreated with 80 μM C2-ceramide (closed circles), or with C2dihydroceramide (open circles). (C) Proliferative response of CD4⁺ T cell clone KS140 to 1 μg/ml of soluble TT and 10 ng/ml TT peptide P2 (residues 830–843) (19). DCs were left untreated (open bars), pretreated with 80 μM C2-ceramide (closed bars), or with C2-dihydroceramide (hatched bars). In all experiments, DCs were pulsed for 1 h with antigen, washed, irradiated, and mixed at 1:4 ratio with T cells. [³H]thymidine incorporation (1 μCi/ well, sp act 5 Ci/mMol) was measured on day 2 and results are expressed as mean of triplicate cultures ± one SD. (D and E) FACS^® histograms of propidium iodide–stained nuclei from untreated DCs (D) or DCs treated with 80 μM C2-ceramide and then cultured for 48 h (E). (F) Adult PBMCs were cultured with different number of allogeneic DCs treated with 80 μM C2-ceramide (closed circles) or left untreated (open squares). [³H]thymidine incorporation were measured after 5 d and results are expressed as mean of triplicate cultures ± one SD.