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. 1996 Dec 1;184(6):2399–2404. doi: 10.1084/jem.184.6.2399

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Expression of PKCδCF results in nuclear fragmentation. HeLa cells were transfected with vectors that express: (A) full-length PKCδ; (B) kinase active PKCδCF; and (C) kinase inactive PKCδCF(K-R). (D) Cells were exposed to 2 μM ara-C. The cells were fixed with paraformaldehyde and then stained with Hoechst dye. Cotransfection of PKCδFL, PKCδCF and PKCδCF(K-R) with pSvβ-β-gal demonstrated transfection efficiencies of 50, 42, and 43%, respectively. Percentage of apoptotic cells for the PKCδFL, PKCδCF, and PKCδCF(K-R) transfected populations was 2, 26, and 4%, respectively. Bar, 15 μM.