Figure 2.

Relative telomerase activity levels of disruptions and compensatory alterations within the pseudoknot domain compared with wild-type telomerase. Telomerase activity levels were normalized by using cell numbers and total protein content of partially purified preparations. The results represent an average of three separate experiments with a minimum of two lines for each sample. (A) Two-base pseudoknot disruptions, 71–72G and 84–85C, and the corresponding compensatory alteration, Comp 1, compared with activity levels for wild-type telomerase (WT). (B) Four-base pseudoknot disruption, 84–87C, and the corresponding compensatory alteration, Comp 2, compared with activity level for wild-type telomerase (WT).