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. 1999 Jun 8;96(12):6621–6625. doi: 10.1073/pnas.96.12.6621

Figure 4.

Figure 4

TER-associated protein complexes separated by native gel electrophoresis. (A) Northern analysis of native complexes. Northern blots probed with either a wild-type-specific end-labeled oligonucleotide (5′-ATTTGAACCTAATTGG-3′) that annealed only to wild-type TER (lanes 1–6) or a mutant-specific end-labeled oligonucleotide (5′-ATTTGGGGGTAATTGG-3′) that annealed only to 84–87C and Comp 2 TERs (lanes 7–12). These antisense oligonucleotides anneal to residues 92–77 of T. thermophila TER. Lane 1 and 7, wild-type; lane 2 and 8, 84–87C; lane 3 and 9, Comp 2; lane 4 and 10, wild-type with 43A template; lane 5 and 11, Comp 2 with 43A template; lane 6 and 12, 84–87C with 43A template. (B) Western analysis of native complexes. Western blots probed with antibody against p95 (lanes 13–18), p80 (lanes 19–24), or p133 (lanes 25–30). Lanes 13, 19, and 25, wild-type; lanes 14, 20, and 26, Comp 2; lanes 15, 21, and 27, 84–87C; lanes 16, 22, and 28, wild-type with 43A template; lanes 17, 23, and 29, Comp 2 with 43A template; lanes 18, 24, and 30, 84–87C.