Table 1.

Detection rate of Brugia malayi DNA in pools of Culex pipiens that initially fed on microfilaremic blood on day 0 and on uninfected blood 7 days later^*

			B. malayi microfilaremic blood†
	Uninfected blood		TaqM		MGB Eclipse
DPI	TaqM	MGB	Non–re-fed	Re-fed‡	Non–re-fed	Re-fed‡
0	0 (1)	0 (1)	100 (5)	NA	100 (5)	NA
10	0 (1)	0 (1)	75 (4)	50 (4)	75 (4)	50 (4)
14	0 (1)	0 (1)	25 (4)	33 (3)	25 (4)	67 (3)

^*Parasite DNA was detected by TaqM or MGB Eclipse real-time polymerase chain reaction. Values are percentage of mosquito pools positive for B. malayi (no. of pools tested). DPI = days post-ingestion of B. malayi; NA = not available.

^†By membrane feeders containing microfilaremic cat blood with 54 MF/20 μl.

^‡Oviposition substrate was provided from 4 to 7 DPI; mosquitoes were then allowed to feed on an uninfected chicken 7 DPI.