Figure 2.
Lateral aggregation of JAM in L transfectants. (a) Immunofluorescence microscopy of JL cells with anti-JAM pAb (C4). JAM was concentrated at cell–cell adhesion sites as planes. (b) Freeze fracture replica image of cell–cell adhesion sites of JL cells. Characteristic intramembranous particle-free domains (*) were frequently observed. (c) The particle-free domains of JL cells were specifically labeled with pAb-specific for the cytoplasmic domain of JAM (anti-JAM pAb, C-tail). Bar, 200 nm.