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. 2001 Aug 6;154(3):491–498. doi: 10.1083/jcb.200103047

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Copyright © 2001, The Rockefeller University Press

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Figure 4. — Interaction between JAM and PAR-3. (a) Recruitment of endogenous PAR-3 to cell–cell adhesion sites in L transfectants. C1L cells, JL cells, or JΔLVL cells were double stained. Claudin-1, JAM, and JAMΔLV were all concentrated at cell–cell adhesion sites. JAM, but not claudin-1–JAMΔLV, recruited PAR-3 to cell–cell contact sites (arrowheads). (b) Six distinct portions of PAR-3 were produced as recombinant fusion proteins with MBP in E. coli, and then the same in vitro binding analysis as described in the legend to Fig. 3 b was performed. Among six types of MBP fusion proteins, only MBP–PDZ1-PDZ3 was bound to GST-JAMcyt. (c) Quantitative analysis of the binding between MBP–PDZ1-PDZ3 of PAR-3 and GST-JAMcyt. K _d value was determined to be 7.5 × 10⁻⁸ M.