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The PX domain can regulate CISK activity. (Top) The activities of wild-type or mutant CISK were compared using luciferase assays. 293T cells were transiently transfected with a mock vector, FKHRL1 (50ng) alone, or FKHRL1 (50 ng) with either wild-type CISK (0.1 or 0.5 μg), CISK-ΔPX (0.1 or 0.5 μg) or CISK-R90A (0.1 or 0.5 μg). Error bars indicate standard errors. (Bottom) The expression of various CISK constructs was examined by Western blotting with an anti-HA antibody.
