Figure 11.

Characterization of cultured SCG neurons. Phase–contrast images of neurons after 3 h (A), 5 d (B), or 21 d (C) in culture. The establishment of cell polarity of SCG neurons after 21 d in culture is demonstrated in D–K. After fixing and permeabilizing, the neurons were labeled with antibodies that recognized the axonal markers tau-1 (D and H) and phospho-M and H neurofilament (E and I) or the somatodendritic markers MAP2 (F and J) and nonphospho-M and H neurofilament (G and K). Two confocal images of each field are shown: D–G have image planes at the level of the cell bodies, whereas H–K show the same neuron at the level of the substratum so that axons can be visualized. Bar, 25 μm.