Figure 2.

The axonal localization of the essential viral membrane protein gB requires Us9 but not gE. Less than 10% of the neurons in a culture were infected for 12 h with the wild-type (A and D), the gE-null mutant (B and E), or the Us9-null mutant (C and F) and then were fixed and permeabilized. Infected neurons were labeled with antibodies that recognize gB. A–C have image planes at the level of the cell bodies, whereas D–F have image planes at the substrate so that axons can be visualized. Arrows point to axons of infected neurons, and N indicates the nucleus of infected neurons. Bar, 25 μm.