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. 2001 Aug 20;154(4):691–698. doi: 10.1083/jcb.200012098

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Copyright © 2001, The Rockefeller University Press

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Figure 5. — Differential distribution of MACF and BPAG1-a/b. Adjacent transverse (A, B, and E–H) and sagittal (C and D) sections of embryonic-day-14.5 embryos were hybridized with probes prepared for the MTBD of BPAG1-a /b (A, C, E, and G) and MACF (B, D, F, and H). Hybridization signal of BPAG1-a was stronger than that of MACF in the DRG (short arrows, A and B) and metanephros (long arrows, A and B). Interestingly, there is a gradient of BPAG1-a/b distribution in the spinal cord with stronger signals detected on the ventral side (A). Note that BPAG1-a mRNA was expressed in the cartilage of the vertebrae, whereas MACF mRNA was expressed mostly in the mesenchymal tissues surrounding the developing vertebrae (C and D). Strong signals for BPAG1-b and MACF were found in the heart (E) and the lungs, respectively (H). Bars: (A and C) 0.5 mm; (H) 0.1 mm.