Figure 1.

E2F and cyclin E/cdk2 collaborate in S phase induction. Analysis of S phase induction with flow cytometry. Cells were starved and infected with Ad-Con (control, moi = 600 ffu per cell), Ad-E2F1 (moi = 100 ffu per cell), Ad-E2F2 (moi = 100 ffu per cell), and Ad-CycE and Ad-Cdk2 (moi = 200 ffu per cell and 300 ffu per cell, respectively) as indicated; the final moi for each sample was adjusted to 600 ffu per cell with Ad-Con. Cells were then incubated with fresh starvation medium or with medium containing 10% serum (+) and then harvested at the indicated times and processed for flow cytometry. The horizontal axis reflects relative DNA content, and the vertical axis represents cell number.