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E2F overexpression induces Mcm protein accumulation. REF52 cells were starved and infected with Ad-Con (moi of 100 ffu per cell) or Ad-E2F2 (moi of 100 ffu per cell) as described. Cells were then incubated with fresh starvation medium or with medium containing 10% serum (control +) and then harvested 24 hr postinfection. Endogenous Mcm2 and Mcm3 proteins were detected by using Western blot analysis with antibodies specific for each protein.
