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. 2001 Jul 9;154(1):71–84. doi: 10.1083/jcb.200101089

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Copyright © 2001, The Rockefeller University Press

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Figure 6. — POM121 is immobilized during nuclear assembly in anaphase. FRAP of a NRK cell transiently expressing POM121-EGFP₃. (A) Boxed area was photobleached to background levels in metaphase. Recovery was monitored every 9 s in a single image acquired with open pinhole. Note rapid recovery of fluorescence into the ER as the cell reaches metaphase/anaphase transition (02:15). (B) FRAP of the same cell as in A ∼6 min after anaphase onset. Note the absence of complete recovery during the transition to telophase. Times, hh:mm:ss. (C) Plot of recovery in the bleached areas shown in A and B for metaphase (green)and anaphase (black). Data was normalized to total loss of fluorescence, time 0 corresponds to the midpoint of the bleach. Note the difference in IFs 4 min after the bleach. Bars, 5 μm.

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