Figure 7.

The amount of transcript produced by the array is correlated with array size. Specimens prepared as in the legend to Fig. 2. Shown in the top row (a–f) are GFP-GR arrays from different cells fixed at 3 h of 100 nM dexamethasone. The corresponding RNA FISH signals are shown in the middle row and the overlay images in the bottom row. Note that progressive increase in array size (a–f) is accompanied by progressive increase in the RNA FISH signal. This correlation is confirmed by quantitative analysis of 113 cells as shown in the plot at the bottom of the figure. Each point in the plot represents an array, like those in panels a–f, whose total RNA FISH intensity has been measured and plotted as a function of the measured perimeter of the array. Bar, 1 μm.