Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2001 Jul 9;154(1):33–48. doi: 10.1083/jcb.200011069

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2001, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 8. — Transcription is required for decondensation. (a) Shown are the percentage of large arrays (>3 μm in length) observed at different DRB concentrations after two different treatment regimens. The data labeled “DRB before Decondensation” come from cells treated simultaneously with 100 nM dexamethasone and different concentrations of DRB. After 2 h, the number of large decondensed arrays was counted. Note that large decondensed arrays decreased significantly in the DRB-treated cells. The cells labeled “DRB after Decondensation” were first treated with 100 nM dexamethasone for 1.5 h to allow arrays to decondense. Then DRB was added at the concentrations indicated, and after a 30–min incubation the percentage of large arrays was determined. Note the significant closing of arrays after the short DRB treatment. Similar results were obtained with α-amanitin. To permit α-amanitin entry, cells were pretreated for 2 h with the drug at the concentrations indicated, and then 100 nM dexamethasone was added for 1.5 h and the number of large arrays was counted. (b) Distribution of array sizes before and after DRB. The effects of DRB on recondensation were not specific to large arrays. Array perimeters were measured from 100 randomly selected cells, each treated with 100 nM hormone for 2 h and then fixed in paraformaldehyde. This distribution was compared with perimeters from cells treated identically except for the addition of 100 μg/ml DRB in the last half hour before fixation. The perimeter distribution after DRB (red, ▪) is shifted to smaller sizes. (c) Comparison of array sizes after DRB to before hormone. A comparable shift in the perimeter distribution occurs when DRB is added with hormone (green curve, ▴). Whether DRB is added with or 1.5 h after hormone, the perimeter distribution (assayed by GFP-GR) resembles that before hormone was added (blue curve, ♦; assayed by DNA FISH). This demonstrates that DRB treatment induces condensation to the prehormone state.