Figure 5.
Telomerase activity in wild-type, PARP-1 +/−, and PARP-1 − /− MEFs. S-100 extracts were prepared from wild-type (A10 and G1), PARP-1+/− (A7), and PARP−/− (A6, G8, and E1) primary MEFs cultures and assayed for telomerase activity. Extracts were pretreated (+) or not (−) with RNase. Protein concentration used is indicated. The arrow indicates the internal control (IC) for PCR efficiency. Same letter refers to littermate embryos.