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1H-NMR spectra of 3-μg HIF (A), ouabain (B), an ouabain sample (10 μg) that had been stored in a borosilicate vial as an aqueous solution (C), and ouabain tetrahedral-borates (D). A and B were measured in 10% acetonitrile-d3 in D2O, whereas C and D were measured in D2O. ∗ indicates the peaks arising from either mechanical noise or because of unidentified impurities.
