Figure 3.

Effect of mutations in the CPF-binding site on CYP7A gene expression. The indicated cell lines were transfected with reporter constructs by using the calcium phosphate method (19). Luciferase assays were performed as described in Materials and Methods. Relative luciferase units were normalized to β-galactosidase activity to control for variations in transfection efficiency. The cross-out refers to the presence of two point mutations (as described in Materials and Methods). The pGL3 reporter is a vector-only control.