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. 1999 Jun 8;96(12):6660–6665. doi: 10.1073/pnas.96.12.6660

Figure 6.

Figure 6

Activation of CYP7A gene expression by CPF. Various expression plasmids and control plasmid pcDNA3 (0.5 μg) were cotransfected into HEK293 cells with 0.5 μg of luciferase reporter plasmid containing the human CYP7A promoter sequence (−716 to +14). (A) Full-length CPF, liver X receptor α (LXRα), retinoid X receptor α (RXRα), and hepatic nuclear factor 4α (HNF4α). (B) Full-length CPF, and its variants. (C) Control plasmid pcDNA3 or an expression plasmid encoding a flag-tagged full-length CPF (0, 0.015, 0.05, 0.15, or 0.5 μg) was cotransfected into 293 cells with the human CYP7A promoter luciferase reporter plasmid. Relative luciferase activity normalized to β-galactosidase activity is shown for each transfection.