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. Author manuscript; available in PMC: 2009 Jan 2.
Published in final edited form as: Mol Cell Endocrinol. 2007 Oct 6;280(1-2):47–62. doi: 10.1016/j.mce.2007.09.011

Figure 6.

Figure 6

RXR agonists do not enhance coactivator recruitment by RXRα/TRβ0 heterotrimers. A. The ability of LG268 to enhance ACTR binding to TRβ0 heterodimers or RXRα/TRβ0 heterotrimers was tested in the absence or presence of T3, using an EMSA supershift protocol similar to that described in Figure 2. The percentage of receptor/DNA complex bound by ACTR (supershifted) was determined under the conditions indicated below the panel. The means of three replicates are presented; error bars depict standard deviations. B. The ability of an ACTR construct to bind to (supershift) TRβ0 heterodimers or heterotrimers in response to LG268 was tested over a range of T3 concentrations, using an EMSA supershift protocol as in panel A. Symbols indicate the means of 3 replicates; error bars depict standard deviations. Curves were fitted using the sigmoidal dose response equation of variable slope from GraphPad Prism 4.0. No statistically significant difference was found between the curves in the presence or absence of LG268 for either heterodimers or heterotrimers.