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Structure of the J15/16 loop in the RNase P RNAs used in this work. The J15/16 loop, connecting helices P15 and P16, is shown from left to right for E. coli, Synechocystis 6803, Anabaena 7120, and Pseudanabaena 6903. The proposed (11) base pairing between the sequence RCCA-3′ in pre-tRNAs and a conserved GGU sequence in J15/16 of E. coli is shown. The GGU sequence mutated in Synechocystis to CCA to generate mutant CCA332 is boxed.
