Table 1.
Effects of ischemia and reperfusion on state 3 respiration and complex activities
| Protocol | n | State 3, nmol of O⋅min−1⋅mg−1 | Complex I, nmol of NADH⋅min−1⋅mg−1 | Complex III, nmol of cytochrome c⋅min−1⋅mg−1 | Complex IV, nmol of O⋅min−1⋅mg−1 |
|---|---|---|---|---|---|
| 8 month old | |||||
| Perfused | 7 | 203.5 ± 26.2* | 136.0 ± 24.5** | 1,921.5 ± 321.3 | 1,993.0 ± 246.7 |
| Ischemic | 6 | 167.0 ± 20.3*‡ | 117.8 ± 6.3**‡‡ | 2,205.4 ± 376.9 | 1,915.5 ± 169.7 |
| Reperfused | 6 | 136.5 ± 22.6‡¶ | 119.7 ± 8.0 | 1,921.5 ± 322.4 | 2,076.3 ± 412.4¶¶ |
| 26 month old | |||||
| Perfused | 6 | 204.2 ± 26.2† | 131.5 ± 17.2†† | 1,969.3 ± 248.2 | 2,019.4 ± 327.9 |
| Ischemic | 6 | 168.3 ± 26.6†§ | 99.5 ± 10.2††‡‡ | 2,195.0 ± 430.0 | 2,135.0 ± 309.5§§ |
| Reperfused | 6 | 110.1 ± 11.7¶§ | 97.3 ± 10.7 | 2,063.6 ± 298.4 | 1,709.4 ± 52.4§§¶¶ |
Perfusion, ischemia, and reperfusion protocols were performed as described in Materials and Methods. Values are expressed as the mean ± SD. P values (determined from paired t test) where like symbols indicate values compared. State 3: ∗, ≤0.009; †, ≤0.02; ‡, ≤0.02; §, ≤0.0003; ¶, ≤0.02. Complex I: ∗∗, ≤0.05; ††, ≤0.001; ‡‡, ≤0.002. Complex IV: §§, ≤0.004; ¶¶, ≤0.03.