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Fractionation procedure for rat liver. A total microsome (TM) fraction obtained by using two centrifugation steps was loaded at the bottom of a sucrose density gradient (sucrose molarities indicated) and centrifuged at 82,000 × g for 3 h. The indicated floating fractions, Golgi light (GL), Golgi heavy (GH), carrier vesicle 1 (CV1), carrier vesicle 2 (CV2), and residual microsomes (RM) were collected. H, homogenate; PNS, postnuclear supernatant; Cyt, cytosol.
