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Protein profile of CCV fractions. (A) CCV fraction (15 μg) separated by SDS/10% PAGE and stained with Coomassie blue. (B and C) Homogenate (H) or CCVs (200 μg) separated on SDS/12% PAGE and immunoblotted for clathrin heavy chain (B) or for GAIP (C). The CCV fraction is enriched for both clathrin and GAIP.
