Figure 3.
(A) Dose-dependent degradation of Ft after (0 h) UVA irradiation of FEK4 fibroblasts as monitored by immunoprecipitation of 35S-labeled FEK4 cells with human Ft polyclonal antibody. Lane 1 represents the nonirradiated control and lanes 2–4 represent samples irradiated with UVA doses of 100, 250, and 500 kJ/m2, respectively. (B) Ft biosynthesis after UVA irradiation of FEK4 cells. (C) HO-2 biosynthesis after UVA irradiation of Fek4 cells. In B and C, the rate of protein (Ft and HO-2, respectively) synthesis was followed by 35S-labeling 2 and 6 h after UVA irradiation of cells with a dose of 250 kJ/m2 (+). Nonirradiated controls are shown as (–). In the 0-h time point, cells were labeled before UVA irradiation.