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. 2001 Mar 5;152(5):959–970. doi: 10.1083/jcb.152.5.959

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Coimmunoprecipitation of KLC and the JIP proteins. Lysates of COS cells expressing Flag-tagged JIP-1, JIP-2, or JIP-3 together with HA-tagged KLC were immunoprecipitated (IP) with no primary antibody (−), with an anti-Flag mAb (F), or with an anti-HA mAb (H). Precipitates were immunoblotted to detect the expressed proteins using polyclonal antibodies to both epitope tags.