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. 2001 Mar 5;152(5):959–970. doi: 10.1083/jcb.152.5.959

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Expression of kinesin dominant negative constructs causes mislocalization of endogenous JIP-1 protein. CAD cells were transiently transfected with plasmids encoding the HA-tagged KLC TPRs (A), HA-tagged PP5 TPRs (B), HA-tagged KLC truncation KLC-176 (C), or Myc-tagged KHC truncation KHC-891 (D). After differentiation, the expressed proteins were detected by indirect immunofluorescence microscopy using mAbs to the epitope tags (left). Endogenous JIP-1 protein was detected with an affinity-purified polyclonal antibody (right). Note that the background fluorescence has been enhanced to show the entire neuronal cell. Arrowheads denote tips of transfected cells; arrows denote tips of untransfected cells.